All reporter assays were carried out in 4 biological replicates. Chromatin immunoprecipitation assay Chromatin immunoprecipitation assays have been per formed during the MDA MB 453 cell line utilizing a ChIP Assay Kit as instructed from the manufacturer. ChIP grade rabbit monoclonal CREB1 antibody was utilized at 4 ?g per assay. To quantify ChIP effects, two primer sets for PIP promoter have been utilized for qPCR amplification making use of the SYBR green technique. Forward primer set. Amplification of input chromatin just before immunoprecipitation at a dilution of one,a hundred was applied being a constructive manage. ChIP assays employing non specific anti physique served as being a adverse handle. The assays have been carried out in 3 replicates and percentage recov ery of input chromatin was calculated for each experimen tal set.
PIP siRNA knockdown PIP knockdown was carried out in MDA MB 453 cells by reverse transfection as described ahead of, utilizing the order CP-690550 following two sets of siRNA oligos, Set one, CREB1 knockdown was carried out applying the next siRNA oligo as described before, Transfection of siRNA oligos working with Lipofectamine RNAiMAX was carried out as instructed from the manufac turer. The ultimate siRNA concentration was 20 nM for that knock down experiments. Cells transfected with siRNA Universal Adverse Handle 1 have been utilized as controls. In all experiments the effects of knockdowns have been assessed seventy two hours immediately after the siRNA transfections. Cell invasion assay Cell invasion assays had been carried out employing CytoSelect 96 Well Cell Invasion Assay Kit as instructed through the producer.
PIP siRNA and handle siRNA transfections have been carried out during the MDA MB 453 cell line as described prior to. Forty eight hrs following the siRNA transfections, cells had been kinase inhibitor Rigosertib har vested and seeded in an invasion assay plate at 50,000 cells/100 ?l per every effectively. Serum cost-free L15 medium and L15 medium with 10% FBS have been utilized for your upper and reduce chambers with the invasion assay plate, respec tively. Up coming, cells were incubated for 24 hours in the 37oC incubator before assaying for invasion. Ultimately, cells had been dissociated in the membrane as per the suppliers instructions and subsequently detected with CyQuant GR Fluorescent Dye. Fluorescence was measured with a fluorescence plate reader at 480 mm/520 mm. Treatment with Purified Human Fibronectin at 100 ?g/ml concentration was carried out 24 hrs immediately after PIP knock down.
Invasion assays have been carried out in three biological replicates. Cell viability assay PIP knockdown in MDA MB 453 cells was carried out as described ahead of. A complete of ten,000 cells transfected with either PIP siRNA or control siRNA have been seeded per nicely of the 96 effectively plate. Seventy two hours just after transfections, cell viability was assessed applying the Vybrant 3 two,five diphenyl tetrazolium bromide Proliferation Assay Kit as instructed by the manufacturer.

Agents were not renewed throughout the total period of cell exposure, and manage cells with no agents had been cul tured under exactly the same ailments with comparable media changes. Following therapy, the media was replaced by drug free of charge medium containing MTT solution, and incubation was prolonged for three h at 37 C. Just after thoroughly getting rid of the supernatants, the formazan crystals formed by meta bolically viable cells were dissolved in DMSO plus the absorbance was established at 570 nm within a multi nicely plate reader. Employing handle optical density values, check OD values, and time zero OD values, the compound con centration that brought about 50% development inhibition was calculated through the equation, 100 ? 50. The information presented are from three separate wells per assay and the assay was carried out no less than three times.
selleckchem EGFR Inhibitor Isobologram examination of drug interactions The interactions of G28UCM and EGCG with anti HER medicines were evaluated by the isobologram system as we have now previously published. Briefly, the con centration of a single agent creating a 30% inhibitory effect is plotted within the horizontal axis, as well as the concen tration of a further agent generating the exact same degree of impact is plotted to the vertical axis, a straight line join ing these two points represents zero interaction involving two agents. The experimental isoeffect factors have been the concentrations from the two agents that when com bined kill 30% on the cells. Once the experimental isoef fect points fell below that line, the combination impact with the two drugs was deemed to become supra additive or synergistic, whereas antagonism happens should the experi psychological isoeffect points lie above it.
Within the created assay assortment, a set of isoeffect factors was produced because there were numerous FASN inhibitors and anti a total noob target agent concentrations that achieved exactly the same iso result. A quantitative index of those interactions was provided from the equation Ix, wherever, for this review, a and b represent the respective concentra tions of FASN inhibitors and anti HER agents needed to produce a fixed level of inhi bition when administered alone, plus a and B signify the concentrations expected for your same effect once the drugs were administered in combina tion, and Ix represents an index of drug interaction. Ix values of 1 indicate synergy, a value of 1 represents addition, and values of 1 indicate antagonism.
For all estimations of Ix, we applied only iso bolos wherever intercept data for each axes have been accessible. Western blot evaluation of tumour and cell lysates Cells and animal tumour tissues were collected and lysed in ice cold lysis buffer containing one mM EDTA, 150 mM NaCl, 100 ug/mL PMSF, 50 mM Tris HCl, protease and phosphatase inhibitor cocktails. A sample was taken for measurement of professional tein content by Lowry based BioRad assay and either utilised instantly or stored at 80 C.

Particularly, decreased H3K27 methylation in activated nave CD4 T cells and in T cells from individuals with SLE was connected with decreased DNA methylation with the IL 17A promoter, together with lowered recruitment of HDAC1 and DNMT3a. These ndings raise the situation that HDACi could have probably excellent and negative eects within the autoimmune ailment setting, specifically with regard to professional inammatory cytokines. Even so, we and other people have also shown that epigenetic targeting agents have the capacity to aect the stability of the two expressed mRNAs and proteins, and this eect has also been observed in RA, in which HDACi signicantly reduced the stability of IL six mRNA in FLSs and macrophages.
It is actually clear through the prior sections that epigenetic targeting agents have the possible to alter or restore expression of important factors in rheumatic sickness or might have the capability to ablate or ameliorate the pro inam matory environments induced by these illnesses. By no means theless, fascinating data have emerged from a phase II clinical trial selleck chemical of givinostat, an orally bioavailable HDACi, in patients with JIA. Within this trial, 17 sufferers have been offered givinostat at a dose of one. five mg/kg every day for as much as 12 weeks. With the end on the trial, a signicant therapeutic benet was observed within the individuals, especially while in the locations of mobility and properly getting. This was coupled by using a lower while in the amount of joints with active arthritis, as assessed from the variety of agonizing and swollen joints. Critically, the security and tolerability prole from the drug was very fantastic, the vast majority of adverse events had been reported as mild or reasonable and consisted of nausea, vomiting, and fatigue.
A second clinical our website trial involving an open label extension of the dose nding research in polyarticular JIA is ongoing, as well as a major end result to determine long term safety is operating. The likely utility of dietary epigenetic targeting agents for rheumatic illness A signicant situation concerning the usage of epigenetic targeting agents in ailments such as rheumatic sickness is they are chronic ailments and demand long-term remedy regimens. Nutrition based mostly interventions, as a result, could offer a novel therapeutic avenue of approach with this particular component in mind. A considerable number of naturally taking place bioactive compounds have been shown to inhibit several members in the epigenetic machinery.
Evidence to link these naturally arise ring compounds with likely patient benet in rheu matic disease is now emerging. One among quite possibly the most extensively studied of these com lbs is curcumin, a all-natural polyphenol happening in turmeric. Conversely, this compound is proven to inhibit the two HDACs and KATs. Two pilot studies are already conducted in sufferers with rheumatic disease. From the rst review, the safety and eectiveness of curcumin alone and in mixture with diclofenac sodium were evaluated in sufferers with lively RA.

sylvestris full genome shotgun project is deposited at DDBJ/ EMBL/GenBank under the accession ASAF00000000. The model described in this paper is version ASAF01000000. The N. entire genome shotgun project is deposited at DDBJ/EMBL/ GenBank under the accession ASAG00000000. The ver sion described on this paper is edition ASAG01000000. The raw sequencing data utilized to the assemblies of N. sylvestris and N. tomentosiformis genomes have already been submitted towards the EBI Sequence Go through Archive beneath accession numbers ERP002501 and ERP002502. Repeat material estimation The repeat material of the N. sylvestris and N. tomen tosiformis genome assemblies have been estimated making use of RepeatMasker together with the eudicot repeat library avail able in the Sol Genomics Network, the TIGR Solana ceae repeat library, and RepeatScout libraries made utilizing sequences of at least 200 kb from the draft genome assemblies of N.
sylvestris and N. tomento siformis. Classification on the repeat varieties was completed utilizing the NCBI BLASTN hits to identified repeat factors. Genetic markers PCR primers for that SSR markers have already been reported previously as well as COSII makers from Sol Geno mics Network have been mapped to your draft assembly gen omes of N. sylvestris and N. tomentosiformis using Last. Only order Imatinib the primer pairs that may be mapped with not less than 95% identity and that yielded a one of a kind PCR pro duct were retained. Pathway gene identification and quantification Genomic regions containing genes that probably encode proteins from the chosen pathways have been identi fied by mapping homologous proteins from other spe cies to the genome assemblies using BLAT and manually curating the hits.
Probes through the Tobacco Exon Array had been chosen by mapping them on the recognized genome areas making use of Last and retain ing only excellent matches that could be mapped uniquely. Quantification of gene expression was obtained by summing the Cufflinks FPKM values from the transcripts that overlapped the identified genome areas. De novo transcriptome ATP-competitive MEK inhibitor assembly Every one of the reads have been preprocessed to clip the overrepre sented sequences reported by FastQC. Soon after clip ping, the three ends from the reads were high-quality trimmed using a good quality threshold of twenty and artifacts had been eliminated. Eventually, reads of at least 50 nucleotides with at the very least 75% nucleotides of excellent 20 or much more have been kept. The clip ping, trimming and filtering were carried out utilizing the fastx toolkit.
Transcripts had been assembled making use of the Trinity de novo assembly pipeline, the peptide pre diction plan contained inside of this computer software suite was utilised to predict peptides from your assembled transcripts. Transcriptome assembly was carried out applying the Tuxedo suite of tools. Reads have been mapped to the appropriate genome assembly applying the Bowtie2/ Tophat2 pipeline with the default parameters. Transcript generation was performed working with the Cufflinks tools and merged applying Cuffmerge.

Notably, none within the above procedures reap the benefits of recent TF microarrays that reveal regulator target genes. Nested effects models are intended to extract regulatory networks from perturbation information, despite the fact that integration of TFBS and gene annotations will not be supported. Nucleosome positioning measurements also continue to be unexplored in all above approaches. In summary, added computational efforts are essential for meaningful integration of versatile biological information. Here we propose a strategy m,Explorer that employs multinomial logistic regression designs to predict professional cess distinct transcription things. We aim to supply the following improvements in comparison to earlier solutions. 1st, our procedure lets simultaneous analy sis of 4 lessons of data, gene expression information, which include perturbation screens, TF binding web sites, chromatin state in gene promoters, and func tional gene classification.
The model is primarily based selleck chemical for the assumption that TF target genes from perturbation screens and TF binding assays are equally informative about TF process specificity. Second, we greatly reduce noise by such as only substantial self-confidence regulatory relation ships, and don’t presume linear relationships involving regulators and target genes. Third, we integrate in depth data to considerably better reflect underlying biol ogy, various subprocesses might be studied in the single model, and chromatin state data are incorporated into TF binding website evaluation. TF target genes with simulta neous proof from gene expression and TFBS information are highlighted separately. Fourth, our analysis is robust to very redundant biological networks, as sta tistical independence is not really necessary.
We use univariate models to research all TFs independently and refrain from over fitting which is characteristic to quite a few model primarily based approaches. This is statistically legitimate underneath the assump tion that a complicated model may perhaps be understood by examining its parts. To check our system, we compiled a complete information set covering most TFs on the budding yeast. We bench marked m,Explorer in the very well read full article studied biological strategy and create its improved effectiveness in comparison to sev eral equivalent methods. Then we used the device to uncover regulators of quiescence, a cellular resting state that serves as a model of chronological age ing. Experimental validations of our predictions unveiled nine TFs with considerable affect on G0 viability.
Moreover demonstrating the applicability of our computational process, these findings are of wonderful potential interest to yeast biologists and researchers of G0 relevant processes like ageing, growth and cancer. Benefits m,Explorer multinomial logistic regression for inferring practice precise gene regulation Right here we tackle the issue of identifying transcription factors that regulate practice unique genes.

Assuming that the mutations will not be mu tually exclusive, this observation implies that the reduction of the PTEN allele only appeared just lately inside the tumor and the bulk of the tumor cells had no detectable somatic occasions in the panel of genes investigated. Finally, the tumor of one patient, also with reduced SDH and higher cellularity, harbored two hallmark mutations at 50% al lelic fraction possibly driving the initial tumor, but carried four mutations at 16% allelic fraction, suggesting the presence of a subclone consist ing of 32% of cells. This research highlights how the dif ferences in allelic fraction observed inside tumors can reveal subclonal populations and genetic drivers, and can be utilized to watch treatment method and probably stop future resistance.
Value with the germline variants Our technique recognized 586 inherited germline variants, which has a median of 140 per patient, 85% of them current in dbSNP. We very first investigated the presence of deleterious variants in BRCA1/2, that are probably the most actionable genes inside the clinical setting. We identified 3 sufferers which has a predicted deleterious mutation in selleck Tosedostat one among these genes, of which only one appears genuinely deleterious. The BRCA1 Q1355 E1356fs frameshift mutation is a previously reported deleterious mutation and it is clinically actionable. Interestingly, the mutant allele was picked for in the tumor, indicating a selective benefit. This germ line acquiring was later confirmed by a Clinical Laboratory Improvement Amendments approved assay soon after the pa tient consulted which has a clinical genetic counselor.
Inherited variants in DPYD are already related with toxicity to five fluorouracil or capecitabine selleck chemical chemotherapy, that is generally utilized in breast cancer treat ment. We recognized 6 individuals carrying three variants in DPYD with predicted deleterious results. 3 pa tients have been heterozygous for rs1801160. This single nucleotide polymorphism defines the DPYD 6 haplotype, which has been associ ated with improved toxicity. Two novel missense variants recognized in three patients have an unknown significance. Interestingly, a recent research signifies that variants in DPYD can basically in crease its metabolic action, therefore guarding towards toxicity and reducing drug efficiency. Till much more practical experiments are performed, it will likely be challen ging to unambiguously establish the clinical relevance of most inherited DPYD variants.
We also identified two sufferers carrying one inactive allele of the gene. However, it’s not clear no matter whether this particu lar allele, in the heterozygous state, is linked by using a re duced metabolic process of tamoxifen, thus, a change in drug dosage is not justified. Much more generally, our strategy recognized a lot of inher ited variants of unknown significance, which needs to be cautiously interpreted.

The corrected and normal ized values for tumor gene expression have been then employed to recognize genes differ entially expressed with respect for the individuals germline plus a compendium of 50 previously sequenced WTSS libraries. This compendium was composed of 19 cell lines and 31 principal samples representing not less than 19 distinct tissues and 25 tumor varieties too as six standard or benign samples. Tumor versus compendium comparisons utilized outlier statistics and tumor versus blood utilised Fishers actual test. We initially filtered out genes with much less than 20% non zero data throughout the compendium. This was necessary to avoid cases where a minor expression worth during the tumor receives an inflated rank when all other libraries reported zero expression. Up coming, we defined above expressed genes as individuals with outlier and Fisher P values 0.
05 and FC for tumor versus compendium and tumor versus blood two and 1. 5, respectively. Very similar procedures had been made use of to define beneath expressed genes. Additionally to lung/skin metastasis versus compendium/normal blood we also in contrast the skin and lung metastases right. Pathway evaluation was selleck inhibitor carried out for all gene lists using the Inge nuity Pathway Analysis computer software. P values for differential expression and pathways analyses have been corrected with the Benjamini and Hochberg procedure. Overlaps had been determined with all the BioVenn net tool. Background A vital aspect of your eukaryotic cell cycle management may be the co ordination of cell cycle progression together with the growth within the cell.
The investigation of this difficulty, extensively studied within the yeasts Saccharomyces SP600125 molecular weight cerevi siae and Schizosaccharomyces pombe, elucidated the essential molecular mechanisms of cell cycle control, which in lots of elements are frequent to all eukaryotes. Genetic research during the yeasts revealed that this co ordination happens at each the G1/S along with the G2/M transitions, with G1/S being the most important stage of handle for S. cerevisiae and G2/M for S. pombe. Useful mutants for defin ing genes concerned while in the fee limiting techniques of these transitions are these that advance cells prematurely into cell division, resulting in cells using a smaller cell dimension than normal. The first of those mutants in fission yeast, wee1 50, was defective in a protein kinase that phosphorylates Tyr15 of the cyclin dependent kinase Cdc2. Phosphorylation of this conserved residue inhibits the CDK, and its dephosphorylation by the phosphatase Cdc25 activates the CDK and triggers mitosis. This posttranslational modification may be the key price limiting management of mitotic onset in fission yeast. Two pathways, the mitogen activated protein kinases stress dietary response along with the cell geo metry sensing pathways, regulate Tyr15 phos phorylation upstream of Wee1 and Cdc25.

4 patients had no mutations, and 34 patients had among one particular and twelve nonsilent mutations. In total, we identified 76 somatic variants across the 34 cases, of which 62 had been nonsilent, leading to a coding modify in 28 genes. To highlight the specificities of the patient cohort and the sequencing assay, we compared our results with those obtained from a considerable TCGA cohort of 507 breast invasive carcinomas that have been sequenced in any way coding genes. We observed that 17% in the TCGA samples had no detectable mutations during the 47 genes of our panel, as in contrast together with the 10% of samples with no de tectable mutations determined by our technique. Similarly, there were 3 or far more somatic muta tions in 18% from the samples in our study in contrast with only 8% within the TCGA dataset.
Thirty nine of your 41 genes mutated either in our research or in the TCGA dataset were mutated during the identical fraction of samples. Only ERBB2 and PMS2 showed a significant dif ference, despite the fact that the substantial difference in sample dimension could weaken this comparison. Altogether, these observations suggest our technique includes a higher sensi selleck chemicals tivity to detect mutations in potentially clinically action able genes. Quite possibly the most often mutated gene, TP53, was altered in 37% from the sufferers. In six individuals, the mutation The 2nd most usually mutated gene, PIK3CA, was mutated in 24% of your sufferers. All of the mutations occurred in mutational hotspots identified to re sult within a phosphoinositide three kinase attain of func tion, E545K, H1047R, E542K and C420R.
In contrast to TP53, the allelic frac tion of PIK3CA mutants was proportional to your tumor cellularity, using the exception of two tumors of substantial cellularity and lower PIK3CA mutant allelic fraction, indicating the mutations LY 2835219 could have been existing in only a subset on the tumor cells. GATA3 was discovered mutated in 16% in the pa tients. Interestingly, five out of the six mutations led to a frameshift, consistent together with the findings of the TCGA and substantially increased compared to the preliminary GATA3 mutational evaluation performed by Sanger sequencing in breast cancer. The frameshift mutations in this transcription issue occurred in the vicinity of your Zn Finger domain, which also sur rounds the nuclear localization signal. The mutations may possibly consequently result in a reduction of function by preventing DNA binding or nuclear import.
The exclusive mutational profile of GATA3, dominated by frameshift mutations, may well prompt further investigations about their mechanism of onset and significance. We also identified less commonly mutated genes with prospective worth from the clinic. 1 patients tumor was de termined to harbor a PIK3R1 K567E mutation, which continues to be observed in endometrial cancer. Although the significance of this particular substitution is just not known, was homozygous, leading to a frameshift, a non sense or perhaps a missense, supporting the complete reduction of perform of TP53 in these circumstances.

In the study examining 113 patient samples covering the total spectrum of EC, main tumor samples exhibited a variable degree of immunoreactivity for CD133/1. Dissociated bulky tumors formed sphere like structures, maintained CD133 expression and may very well be propagated for up to 12 weeks. CD133 cells purified from endometrioid adenocarcinomas have been resistant to cisplatin and paclitaxel induced cytotoxicity and expressed a peculiar gene signature consisting of high levels of matrix metalloproteases, interleukin eight, CD44, and CXCR4. When serially transplanted into NOD/ SCID mice, CD133 cells had been capable of initiating tumor formation and recovering the phenotype with the unique tumor. Markers ALDH1 was detected within a smaller population of endomet rial tumor cells that had been much less mature.
ALDH1 optimistic cells were much more tumorigenic, resistant to anticancer agents, and more invasive kinase inhibitor DMXAA than ALDH1 adverse or lower cells. Clinically, large degree of ALDH1 was correlated with lymphatic invasion, recurrence, and poor prognosis of individuals. ALDH1 is really a candidate CSCs marker for uterine endometrioid adenocarcinoma. Msi one was immunolocalized to single epithelial cells and modest clusters of stromal cells in endometrial, endometriotic and endometrial carcinoma tissue specimens. Msi one cells have been mostly located during the basalis in the proliferative stage in the menstrual cycle, suggesting their doable stem/progenitor cell perform. Stromal Msi 1 cells have been not discovered in a perivascular area, though some have been inside a periglandular area, a similar area to some stromal LRCs in mouse endometrium.
A considerable proportion of endometriotic glands expressed Msi one. Immunofluorescence microscopy unveiled colocalization of Msi one with its molecular target Notch 1 and telomerase. In endometrium, MSCA one, a bone marrow derived MSC surface marker identified as tissue nonspecific alkaline phosphatase, is expressed straight from the source at inter mediate levels on CD146 cells and at high levels while in the luminal room of glandular epithelia. In conclusion, human endometrium also harbors a rare population with MSC and fibroblast properties that can be par tially purified as a CD146, PDGF RB population. A larger proportion on the rare these cells differen tiate compared to unfractionated fibroblasts, and in dividual CFU are definitely multipotent as their progeny differentiate into numerous mesodermal lineages. This suggests that a hierarchy exists within the MSC fibroblast lineage. Obviously this requirements even further molecular, genetic and in vivo characterization. Cervical cancer stem cells Markers Although limited, proof supporting the existence of stem like cells in cervical tumors is convincing.

Bromophenol blue and Tris base were from Carl Roth, Karlsruhe, Germany, and sodium dodecyl sul fate was from Serva, Heidelberg, Germany. Gly cerin, potassium ferricynaide and sodium thiosulfate had been from Merck, Darmstadt, Germany and formic acid from BASF, Ludwigshafen, Germany. Superoxide dismu tase 2 antibodies was a gift from Dr. Dihazi, UMG, Goettingen, Germany. b tubulin antibody was from BioVendor, Heidelberg, Germany and antibodies to HRP labelled anti mouse secondary antibodies were from Bio Rad, Munich, Germany. Cell cultures Human T lymphoblastic leukaemia cells had been bought from DSMZ. Cells were grown in 75 cm2 culture flasks in RPMI 1640 med ium containing L glutamine, 10% FCS, one hundred,000 U/L penicillin and a hundred ug/L streptomycin, in 95% humidity and 5% CO2 ailments at 37 C.
Heat inactivation and LPS remedy of cultured cells additional info FCS was heated at 56 C for thirty minutes prior to including it to your RPMI 1640 medium. CCRF CEM cells have been grown in RPMI 1640 medium supplemented both with FCS with out heat inactivation along with a usual concen tration of LPS, FCS with heat inactivation containing a usual concentration of LPS, FCS without having heat inactivation acquiring a very low concentration of LPS, or heated FCS with low concentration of LPS. The cells have been adapted in RPMI 1640 medium supplemented with four unique FCS concen trations for no less than 5 passages ahead of starting the very first harvest. The cells had been grown to a density of 0. 25 ? 106 cells/mL under suggested circumstances i. e, 37 C, 95% humidity, 20% O2, 5% CO2 along with the medium was chan ged every single 2nd day.
All experiments had been repeated six times. selleck inhibitor Cell lysis and protein estimation Cells were washed with ice cold PBS and lysed in lysis buffer. Protein concentration was measured as described by Bradford applying serum albumin like a common. Sample planning and two dimensional gel electrophoresis two DE was carried out as described by Gorg et al. Briefly, a 160 ug protein sample was diluted in rehydra tion buffer have been utilized on immobilized pH gradient strip using a non linear pH variety of 3 10 at area temperature overnight for passive rehydration. Iso electrical focusing was performed that has a Bio Rad Protean electrophoresis apparatus set to final 32000 Volts hour. The IPG strip was then equilibrated for twenty minutes in equilibration buffer containing DTT and then subsequently immersed for 20 minutes in fresh equilibration buffer containing iodoacetamide. Following equilibration, proteins have been separated by SDS Web page at a consistent voltage of one hundred Volts utilizing a twelve. 5% polyacrylamide separation gel at 4 C. Phospho precise staining of two DE gels The gels were fixed twice in alternative containing 50% methanol and 10% acetic acid for 45 minutes and washed three occasions in double distilled water for 15 min utes just about every.