The validity and reliability of the proposed selleck kinase inhibitor method was assessed by the recovery studies and was summarized in Table 1. Further, the validity and reliability of the proposed method were also accessed by the standard addition method [Table 3]. These results revealed that any minor change in disodium edetate concentration in solutions could be accurately determined by the proposed analytical method. The low values of the standard error (SE) of slope and intercept [Table 1] indicated high precision of the proposed method. Precision was also determined by studying the repeatability and intermediate precision. Repeatability was found in range of 19.96�C29.92 ��g/mL at all the given levels of disodium edetate concentrations [Table 4]. In an intermediate precision study, %RSD values were found to be less than 2% in all the cases.
The RSD values found were well within the acceptable range indicating that the proposed method has an excellent repeatability and intermediate precision [Table 4]. These results also suggested that the proposed method may be considered validated in term of precision. Table 4 Precision data for the developed method Limit of detection and limit of quantitation LOD and LOQ of calibration curve were calculated which was based on the standard deviation (��) of y-intercept of regression line and slope (S) of the calibration curve at the levels approximating the LOD and LOQ, LOD = 3.3 ��/S and LOQ = 10 ��/S respectively. LOD and LOQ of calibration curve were found to be 1.190 and 3.608 ��g/mL, respectively for disodium edetate [Table 1].
CONCLUSIONS It is concluded from the performed study that the developed UV-spectrophotometric method for the estimation of disodium edetate in topical gel formulations, is a simple and cost-effective method. Results also showed good precision and reproducibility. It showed acceptable linearity and accuracy. The proposed method is found to be highly sensitive; therefore, it could be used for routine analysis of disodium edetate in topical gel formulations. Footnotes Source of Support: Nil Conflict of Interest: None declared.
Chromatography was performed on aluminum-backed silica gel 60F254 HPTLC plates (10 �� 10 cm) prewashed with methanol; plates were developed with acetonitrile-methanol-0.1% acetic acid (3.5:2.6:3.9, v/v) in a Camag twin�Ctrough chamber (10 �� 20). Standard solutions of losartan potassium were transferred to different 10 ml volumetric flask and diluted to volume with the methanol such that Cilengitide the final concentrations of losartan potassium were 5.0-30.0 mcg/ml. Both standards and samples (0.3 ��l) were applied to the plates as 6 mm bands by means of a Camag Linomat IV sample applicator.