7 but < 2 enabled the identification at the genus level; and a sc

7 but < 2 enabled the identification at the genus level; and a score < 1.7 did not enable any identification. For strain ph1T, the maximal obtained score was 1.25, thus suggesting that our isolate was not a member of a known species. We added the spectrum from strain ph1T to our currently database for future reference (Figure 4). Finally, the gel view allows us to highlight the spectra differences with other of Peptoniphilus genera members (Figure 5). Figure 4 Reference mass spectrum from P. obesi strain ph1T. Spectra from 12 individual colonies were compared and a reference spectrum was generated. Figure 5 Gel view comparing Peptoniphilus obesi ph1T spectra with other members into Peptoniphilus genera (Peptoniphilus timonensis, Peptoniphilus senegalensis, Peptoniphilus grossensis, Peptoniphilus ivorii, Peptoniphilus indolicus, Peptoniphilus harei, Peptoniphilus .

.. Genome sequencing and annotation Genome project history The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA similarity to other members of the genus Peptoniphilus, and is part of a study of the human digestive flora aiming at isolating all bacterial species within human feces. It was the seventh genome of a Peptoniphilus species and the first genome of P. obesi sp. nov. A summary of the project information is shown in Table 3. The Genbank accession number is “type”:”entrez-nucleotide”,”attrs”:”text”:”CAHB00000000″,”term_id”:”390160866″,”term_text”:”CAHB00000000″CAHB00000000 and consists of 32 contigs arranged in 5 scaffolds. Table 3 shows the project information and its association with MIGS version 2.

0 compliance. Table 3 Project information Growth conditions and DNA isolation P. obesi sp. nov. strain ph1T(CSUR=P187, =DSM=25489), was grown anaerobically on 5% sheep blood-enriched BHI agar at 37��C. Four petri dishes were spread and resuspended in 3×500 ��l of TE buffer and stored at 80��C. Then, 500 ��l of this suspension were thawed, centrifuged for 3 minutes at 10,000 rpm and resuspended in 3��100��L of G2 buffer (EZ1 DNA Tissue kit, Qiagen). A first mechanical lysis was performed by glass powder on the Fastprep-24 device (Sample Preparation system, MP Biomedicals, USA) using 2��20 seconds cycles. DNA was then treated with 2.5 ��g/��L lysozyme (30 minutes at 37��C) and extracted using the BioRobot EZ1 Advanced XL (Qiagen).

The DNA was then concentrated and purified using the Qiamp kit (Qiagen). The yield and the concentration was measured by the Quant-it Picogreen kit (Invitrogen) on the Genios Tecan fluorometer at 37.2 ng/��l. Genome sequencing and assembly DNA (5 ��g) was mechanically fragmented on Brefeldin_A a Hydroshear device (Digilab, Holliston, MA,USA) with an enrichment size of 3-4kb. DNA fragmentation was visualized through an Agilent 2100 BioAnalyzer on a DNA labchip 7500 with an optimal size of 3.287kb. The library was constructed according to the 454 GS FLX Titanium paired-end protocol.

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