We demonstrate that c-EUS

is a sensitive and precise tool for ampullary tumor staging. Conclusion: C-EUS is a high-precision method for preoperatively staging ampulla of Vater tumors, having a high level of correlation with resected specimens. At present time, new c- EUS equipment operates with frequencies between 5; 10 and 12 MHz learn more allowing a better resolution of duodenal wall, which optimizes T staging. Curvilinear endoscopic ultrasound assessment is fully comparable to radial echoendoscopy with the advantage of having the capability of diagnosis, staging, and Fine Needle Aspiration if the case requires. Key Word(s): 1. curvilinear EUS; 2. ampulla of Vater; 3. neoplasia; Presenting Author: JIU-HONG MA Additional Authors: XI HUANG Corresponding Author: JIU-HONG MA Affiliations: Digestive endoscopic center of the first affiliated hospital of Nanchang University,; Digestive endoscopic center of the first affiliated hospital of Nanchang University Objective: A number of different types of endoscope cleaning brushes are available commercially but to date no studies have been published showing their comparative effectiveness in terms of Pseudomonas Aeruginosa removal. To evaluate and compare the removal effectiveness of different endoscopic cleaning brushes and times which are commonly used in endoscopic reprocessing

procedure against LY2606368 research buy Pseudomonas Aeruginosa on endoscopic lumen. Methods: Pseudomonas Aeruginosa was generated on 50 cm endoscopic lumens under low flow conditions for 10 min. The tube was washed in sterile phosphate buffered saline solution 3 times to remove any planktonic bacteria and then randomly brushed by different lumen-cleaning devices. Each device was further separated into groups with designated brushing times of once, twice, and three times. After the brushing step, PAK5 the tubes were further immersed in phosphate buffered saline solution to wash away remaining planktonic bacteria. The bacteria viable count was performed and serial 10-fold dilutions were made. Results: The between-subjects effects were found in two variables: brush devices and brushing times. Overall significant differences in residual bacteria were observed among the different

brush devices and number of brushings. Therefore, separate estimates of the effect of each variable were obtained. Multiple comparisons showed that significant differences existed between control group and brush groups. Compared wiper brush with stiff wire brush, wiper brush had a better effect. Conclusion: We found that two different types of cleaning brush resulted in removal of Pseudomonas Aeruginosa to some degree. It is critical that any guidelines must emphasize that manual cleaning is a critical part of the endoscope cleaning and disinfection process as only mechanical cleaning can prevent residues in the interior channels from remaining after the disinfection process. In our study, the wiper brush was marginally superior to the stiff wire brush.