Treatment of inflammation was initiated an hour after induction with croton oil and the reduction in oedema was measured after 3 h ( Fig. 1, left panel) and 6 h ( Fig. 1, right panel) with (R)-5 and (S)-5. After 3 h treatment, diclofenac inhibited oedema by 55.7 ± 8.4%. Compound (R)-5 was the least active (50.1 ± 4.2%), whilst compound (S)-5 and the racemate exhibited slightly higher activities (58.9 ± 4.0% and 60.0 ± 2.5% respectively). The difference in activity between (R)-5 and the racemate was significant

(P < 0.05). After 6 h treatment, the activity of diclofenac, (S)-5 Selleckchem Quisinostat and the racemate decreased significantly, suggesting a relatively short duration of action. The difference in activity of (R)-5 between 3 and 6 h was the least significant (P > 0.05). After 6 h treatment, diclofenac was the least active (34.7 ± 7.2%; P < 0.001), followed by (S)-5 (39.0 ± 4.6%; P < 0.05), (R)-5 (40.1 ± 8.4%) and the racemate (42.4 ± 4.0%; P < 0.01). Cytotoxicity is an important factor to consider when testing for any biological activity. The in vitro cytotoxicity of the compounds were tested in mammalian MK-8776 mouse cells and compared to diclofenac and

the known cytotoxic drug emetine. IC50 values are represented in Table 1. Diclofenac was the least toxic, followed by (R)-5, (S)-5 and the racemate. The racemate was approximately 10-fold more toxic than (S)-5, and approximately 20-fold more toxic than (R)-5. This difference in cytotoxicity profiles may indicate interactions with different receptor systems. In conclusion, (R)-5 which is naturally found does provide the best therapeutic option in terms of a favourable cytotoxicity profile. The varying anti-inflammatory activities and cytotoxicity profiles seem to suggest that (R)-5 and (S)-5 does

Methisazone not share the same mechanism of action. All authors have none to declare. We acknowledge the University of KwaZulu-Natal Competitive Research Fund, NRF (Gun RH-6030732) and Rolexsi (Pty) Ltd for financial support. We also thank Ms Sithabile Buthelezi and Mr Dennis Ndwandwe for experimental assistance. “
“National Nanotechnology Initiative (NNI) define nanotechnology as the consumption of structures with at least one dimension of nanometer size for the production of materials, systems or devices with initially or extensively improved properties due to their nano size. Since nano-particles have high surface energy and a large surface area-to-volume ratio, it can provide high durability for fabrics, at the same time presenting good affinity for fabrics and enhance durability of the function. Nano-Tex known as a secondary of the US-based Burlington Industries have done the earliest work on nanotextiles.1 To apply nano-particles onto textiles, the most frequently used Modulators technique is coating. Textiles are generally composed of nano-particles; a surfactant, ingredients and a carrier medium to entrap the nano-particles.2 Spraying, transfer printing, washing, rinsing and padding are the several methods can apply coating onto fabrics.