“OBJECTIVE: An in vitro study was carried out to evaluate the effect of curcumin, lycopene, and irradiation upon oral squamous cell carcinoma (OSCC).
MATERIALS AND METHODS: Curcumin and lycopene were administrated at doses of 3, 4.25, 5.50, and 6.75 mu M in PE/CA-PJ15 OSCC cultures irradiated with different doses (1, 2.5, and 5 Gy), followed by evaluation of the effects upon cell viability, apoptosis, and migration after 24, 48, and 72 Selleck PF477736 h of incubation.
RESULTS: The application of curcumin or lycopene to the tumor cells during 24, 48, and 72 h without irradiation exerted an inhibitor effect upon cell viability and increased cell apoptosis. The maximum reduction in cell viability and the peak apoptotic
effect was recorded with the 5.50 and 6.75 mu M doses, for both curcumin and lycopene. Likewise, curcumin and lycopene exerted a synergic effect upon both variables on applying irradiation. Lastly, the 5.50 and 6.75 mu M drug doses, together with 5 Gy of irradiation, yielded the greatest
decrease in cell migration capacity with both curcumin and lycopene.
CONCLUSIONS: Curcumin and lycopene increase cytotoxic activity in the PE/CA-PJ15 cell line and reduce cell migration capacity, GSK126 mechanism while the combination of curcumin or lycopene with irradiation exerts a synergic effect.”
“Our search for biologically active marine natural products led to the isolation of two new ceramides, iotrochotamide I (1) and iotrochotamide DAPT II (2), together with three known 6-bromoindole alkaloids (6-bromo-1H-indole-3-carbaldehyde (3), 6-bromo-1H-indole-3-carboxylic acid methyl ester (4), and 6-bromo-1H-indole-3-carboxylic acid ethyl ester (5)) from the sponge Iotrochota purpurea collected in Indonesia. The structure elucidation of these compounds was secured by spectroscopic methods (1H, 13C, DEPT, COSY, HMQC and HMBC), accurate mass measurements (ESI, EI and GS-MS) as well as comparison with known compounds.”
“The Kidd blood group system consists of three common phenotypes: Jk(a+b-), Jk(a-b+) and Jk(a+b+), and one rare phenotype, Jk(a-b-). Jk(a)/Jk(b) polymorphism is associated with c.838G>A (p.Asp280Asn) in exon 9 of the
JK (SLC14A1) gene, and the corresponding alleles are named JK*01 and JK*02. The rare phenotype Jk(a-b-) was first found in a Filipina of Spanish and Chinese ancestry, and to date, several JK null alleles responsible for the Jk(a-b-) phenotype have been reported. We report seven novel JK null alleles, 4 with a JK*01 background and 3 with a JK*02 background, identified from Jk(a-b-) Japanese.”
“Two new metabolites, pyrenocine J (1) and pyrenochaetic acid D (2), together with two known metabolites, pyrenocine A (3) and pyrenochaetic acid A (4), were isolated from a soil fungus, Curvularia affinis strain HS-FG-196. Their structures were established by extensive spectroscopic analysis. Compound 1 showed cytotoxic activity against the human hepatic cancer cell line HepG2 with an IC50 value of 28.5 mu g/ml.