Methods: Serum levels of CypB in gastric cancer patients and healthy volunteers were measured by ELISA. The expression patterns of CypB were observed in gastric cancer and adjacent non-tumor tissue using immunohistochemistry. MTT, colony formation and cell cycle assays were used to examine the effects of knock-down CypB on the cell growth, proliferation. DNA aptamers specific to CypB were islolated
by SELEX. Binding affinity (Kd) was determined in direct binding assay by flow cytometry analysis. Results: CypB is over-expressed in both serum and tissues of gastric cancer patients. Knock-down of CypB can inhibit gastric cancer cell NVP-BKM120 molecular weight growth, proliferation, cell cycle progress and tumorigenesis. Two aptmers which can bind to CypB protein with high affinity and specificity were isolated through SELEX. Conclusion: These findings indiccate CypB could be a potential biomarker and therapeutic target for MLN8237 chemical structure gastric cancer. CypB-specific aptamers were succusfully isolated, which may be used in further study and future application of CypB. Key Word(s): 1. CypB; 2. gastric cancer; 3. aptamer; 4. SELEX; Presenting
Author: WANG AIYING Additional Authors: ZHU DAN Corresponding Author: WANG AIYING Affiliations: Peking University Third Hospital Objective: This study was to evaluate expression of Bax, Cyt- C and Ki – 67 in DMH induced rat small intestinal and colorectal tumor. Methods: 7 small tumors and 28 colon tumors specimens from 25 male Wistar rats induced by DMH, 9 control only rats, were detected by 上海皓元医药股份有限公司 Pathology and immunohistochemistry detection. The immunohistochemical detection expression of Bax, Cyt −C and Ki – 67 in the tumor and normal tissue, the statistical processing using chi-square test. Results: (1) the positive expression of Bax in the normal tissue and tumor of small intestine were 88.89% and 14.29% respectively; (2) positive expression of Bax in normal tissue and tumor of colon were 44.44% and 89.29% respectively; (3) Cyt – C positive expression
were 100% and 42.86% in normal small intestine and tumors; (4) Cyt C positive expression were 88.89% and 32.14% respectively in normal colon and tumor; (5) normal intestine without positive expression of Ki – 67, small intestine tumor 71.43% positive expression; (6) positive expression of Ki – 67 was 22.22% in normal colon, and 67.86% in colon cancer. Above all have significant difference between groups (P < 0.05). Conclusion: Cyt-C down regulation and Ki- 67 up regulation may promote the occurrence of small and large intestinan tumor. In the normal small intestinal tissue, the expression of Bax was higher than normal colon tissues, Cyt – C high expression and no expression of Ki – 67, may explains the significant difference in tumor incurrence between small intestine and colon. Key Word(s): 1. intestinal tumor; 2. Bax; 3. Cyt-C; 4.