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Competing interests The authors declare that they have no competing interests. Authors’ contributions RB-M performed the experiments and wrote the manuscript. AJC carried out the viral infections and titrations. ET and AA participated in the experimental design and helped to edit the manuscript. JAL-G and AF-R conceived and designed the study, and participated in experimental design. JAL-G coordinated the study and edited the manuscript. All authors read and approved the final manuscript.”
“Background Polyhydroxyalkanoates (PHA) are intracellular storage materials of carbon and energy in many prokaryotes. Ralstonia eutropha is the most prominent and best-studied poly(3-hydroxybutyrate (PHB) accumulating bacterium [1–3]. The results of 25 years of research on biosynthesis, maintenance, intracellular degradation (mobilization) and application of PHA meanwhile provide a good picture on the structure and components of PHB granules. PHB granules are composed of an amorphous polymer core that is enclosed by a dense proteinaceous surface layer (for reviews see [4–13]). Polymer and surface layer constitute a multifunctional complex for which the term carbonosomes has been proposed [14].