Methods: Fifty full-term infants with neonatal jaundice
were included. All patients were subjected to a routine hematologic evaluation, total and indirect serum bilirubin levels, direct Coombs test, qualitative evaluation of G6PD enzyme activity, and detection of G6PD Mediterranean and silent mutations by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.
Results: There were 21(42%) patients who were G6PD deficient; 29 (58%) had normal activity of the enzyme. Among the G6PD deficient patients, 7/21 (33.3%) had Mediterranean mutation and 6/21 (28.6%) had silent mutation.
Conclusion: Our findings, together with other reports, provide data on the prevalence of the Mediterranean variant in Egyptian infants. Further characterization of the G6PD variants requires molecular studies, including analysis of the entire G6PD coding sequence.”
“Objective: To test the fluorescent brightening ATM/ATR inhibitor (FB) staining
method, in which fungi are grown on cellulose nitrate filter membranes, dried, and rendered transparent with oil after staining with lactophenol cotton blue and to encourage use of FBs to eliminate the need for drying and clearing the membranes, therefore simplifying the procedure and reducing the time to perform it.
Methods: Uvitex 2B (U2B) and 9 calcofluor white (CFW) reagents Selleckchem Metabolism inhibitor were used with 25 genera or species of fungi grown on nitrocellulose (NC), nylon (N), and polyvinyl chloride (PVC) filter membranes placed on a variety of growth media. For microscopic examination, membranes were removed from the surface of the growth medium and placed on. microscope slides, with a drop of FB added to each; the preparations were then coverslipped and examined via violet light epi-illumination.
Results: With many of the fungi tested, if growth was not completely inhibited, it was restricted on the PVC compared to that on the NC and N membranes and directly on a medium surface. The fluorescence of fungal structures and their contrast with the background were favorable with all 3 types of membranes and all the FBs tested. For staining https://www.selleckchem.com/products/apo866-fk866.html yeasts on membranes from media containing polysorbate 80 (TWEEN 80), U2B is preferable to CFW because
TWEEN 80 interferes with CFW staining.
Conclusion: FB staining of fungi grown on NC or N membrane filters provides a rapid and simple method to examine fungal growth, especially young cultures, with minimal disturbance.”
“The present study was designed to explore the total flavonoid and taxifolin contents and the radical scavenging activity of 50% ethanol extracts of Polygonum orientale leaf, stem and seed by 2,2-diphenyl-1-picrythydrazyl (DPPH) assay. The extract with higher total flavonoid content has higher radical scavenging activity. Taxifolin (IC50 = 2.83 mu mol/L) has stronger antioxidant activity than that of rutin (IC50 = 3.08 mu mol/L). The free radical scavenging potential of the chloroform, ethyl acetate, water, ethanol and methanol extracts of P.