Appl Environ Microbiol 2001,

67:1581–1586.PubMedCrossRef 35. van Eldere J, Janssen P, Hoefnagels-Schuermans A, van Lierde S, Peetermans WE: Amplified-fragment length polymorphism analysis versus macro-restriction fragment analysis for molecular typing of Streptococcus pneumoniae isolates. J Clin Microbiol 1999, 37:2053–2057.PubMed 36. Lopes MM, Silva D, Freitas G, Tenreiro R: Simultaneous identification and typing of Candida species by MSP-PCR and AFLP: study of clinical isolates from a Portoguese pediatric hospital. Med Mycol 2007, Fer-1 manufacturer 17:157–167. 37. Savelkoul PH, Aarts HJ, de Haas J, Dijkshoorn L, Duim B, Otsen M, Rademaker JL, Schouls L, Lenstra JA: Amplified-fragment length polymorphism analysis: the state of an art. J Clin Microbiol 1999, 37:3083–3091.PubMed 38. Lott TJ, Kuykendall RJ, Welbel SF, Pramanik A, Laser BA: Genomic heterogeneity in the yeast Candida parapsilosis TPCA-1 . Curr Genet

1993, 23:463–467.PubMedCrossRef 39. Fundyga RE, Kuykendall RJ, Lee-Yang W, Lott TJ: Evidence for aneuploidy and recombination in the human commensal yeast Candida parapsilosis . Genetics and Evolution 2004, 4:437–443. 40. Garcia-Effron G, Katiyar SK, Park S, DNA Damage inhibitor Edlind TD, Perlin DS: A naturally occurring proline-to-alanine amino acid change in Fks1p in Candida parapsilosis , Candida orthopsilosis , and Candida metapsilosis accounts for reduced echinocandin susceptibility. Antimicrob Agents Chemother 2008, 52:2305–2312.PubMedCrossRef 41. Tsang LH, Cassat JE, Shaw LN, Beenken KE, Smeltzer MS: Factors contributing to the biofilm-deficient phenotype of Staphylococcus aureus sarA mutants. PLoS One Selleckchem Fluorouracil 2008, 3:e3361.PubMedCrossRef 42. Boles BR, Horswill AR: Agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS Pathog 2008, 4:e1000052.PubMedCrossRef 43. Martí M, Trotonda MP, Tormo-Más MA, Vergara-Irigaray M, Cheung AL, Lasa I, Penadés JR: Extracellular proteases inhibit protein-dependent biofilm formation in Staphylococcus aureus . Microb Infect 2010, 12:55–64.CrossRef

Authors’ contributions AT designed the study with LAMH, performed phenotypical analysis and drafted the manuscript; LAMH conceived the study with AT, performed AFLP analysis and wrote the manuscript; SM participated in the drug susceptibility assays; LM has made substantial contribution to acquisition of data and critically revised the manuscript. SS participated in the study coordination and has made substantive contribution to data analysis; MC participated in the study design and has given the final approval to the version to be published. All authors have read and approved the final version of the manuscript.”
“Background Chlamydiae are implicated in a wide variety of diseases in both animals and humans.