Capsaicin, or its Vehicle, was directly administered into the masseter muscle and changes in resting SHP099 cell line discharge, responses to mechanical stimulation on the cutaneous receptive field and the electrical threshold for masseter nerve stimulation were assessed. Intramuscular capsaicin induced significant increase in the background discharge and mechanical hypersensitivity to the cutaneous stimulation and lowered the threshold for masseter nerve stimulation-evoked responses in the majority of neurons. The capsaicin-induced increase in evoked responses, but not the resting discharge, was partially attenuated when the muscle was pretreated with a mGluR antagonist. The present
study suggests that injury or inflammation in the masseter muscle induce generalized hyperexcitability of central trigeminal neurons and that the blockade of peripherally localized mGluR5 can effectively attenuate muscular hypersensitivity. (C) 2009 Elsevier Ireland Ltd. All rights reserved.”
“In order to determine sources and metabolism of melatonin in human cerebrospinal fluid (CSF), melatonin and 6-sulfatoxymelatonin
(aMT6S) concentrations were measured in CSF sampled during neurosurgery in both lateral URMC-099 mouse and third ventricles in patients displaying movement disorder (Parkinson’s disease, essential tremor, dystonia or dyskinesia) and compared with their plasma levels. Previous determinations in nocturnal urine had showed that the patients displayed melatonin excretion in the normal range, compared with healthy controls matched according to age. A significant difference in melatonin concentration was observed between lateral and third ventricles, with the highest levels in the third ventricle (8.75 +/- 2.75 pg/ml vs. 3.20 +/- 0.33 pg/ml, p = 0.01). CSF aMT6s levels were similar in both ventricles and of low
magnitude, less than 5 pg/ml. They were not correlated with melatonin levels or influenced by the area of sampling. Melatonin levels were significantly higher in third ventricle than in the plasma, whereas there was no difference between plasma and lateral ventricle levels. These findings show that melatonin may enter directly the CSF through the pineal recess in humans. The Tideglusib molecular weight physiological meaning of these data remains to be elucidated. (C) 2009 Published by Elsevier Ireland Ltd.”
“Noise-induced hearing loss (NIHL) has been thought to primarily involve damage to the sensory hair cells of the cochlea via mechanical and metabolic mechanisms. This study examined the effects of baicalin, baicalein, and Scutellaria baicalensis (SB) extract against NIHL in a mouse model. Mice received oral treatment with SB, baicalin, baicalein beginning 30 min prior to noise exposure and continuing once daily throughout the study. Hearing threshold shift was assessed by auditory brain stem responses for 35 days following noise exposure.