Chlorogenic acid is one of many most abundant dietary polyphenols that’s diverse biologic activities including anti Lonafarnib price activity, antioxidant activity, anti carcinogenic activity, anti sensitive activity, modulating activity of cytochrome P450linked enzyme, and apoptosis inducing activity in human oral squamous cell carcinoma and salivary gland tumefaction cell lines. Within our earlier in the day research, we noted that Chl mediated inhibition of BcrAbl phosphorylation results in apoptosis of Bcr Abl CML cells. ROS play an important physiological role as secondary messengers and restrict the appearance of several genes and signal transduction pathways. The redox metabolism that maintains the homeostasis of ROS is crucial in regulation of cell death and in cell signaling. On one hand, low levels of ROS may promote cancer by transforming typical cells through activation of transcription factors or inhibition of tumor suppressor genes, on the other hand, increased ROS levels prevent cancer development through the stimulation of pro apoptotic signs, leading to the death of cancer cells. Therefore, ROS exert a peculiar effect on cancer cells. Cyst cells have higher quantities of ROS than their standard counterparts and are therefore more sensitive and painful to the additional oxidative stress produced by anticancer agents. Emerging evidence suggests that ROS induce programmed cell death in several cancer cells. Recently, many substances like adaphostin, arsenic trioxide, t phenylethylisothiocyanate have been shown to induce Retroperitoneal lymph node dissection apoptosis in Bcr Abl cells by the generation of ROS. A recent study from our laboratory revealed that Chl uniquely induced apoptosis of Bcr Abl CML cell lines and primary cells from CML patients in vitro in a time and dose dependent manner and lowered xenografts of Bcr Abl CML cells in nude mice. Chl inhibited Bcr Abl phosphorylation and induced p38MAPK dependent apoptosis in these cells. Plant polyphenols usually are considered to be anti-oxidants, but they also present prooxidant homes. Many free radical scavengers act in oxidation reduction reactions which are reversible, PF 573228 and some, such as for instance phenolic phytochemicals, according to their design and the conditions may act both as anti-oxidants and prooxidants. A recent report suggested the prooxidant house of Chl. Here, we examined whether Chl induced downregulation of Bcr Abl phosphorylation accompanied by activation of downstream signaling pathways that ultimately cause cell death are consequences of improved generation of intracellular ROS. Chlorogenic acid was purified as previously explained from leaves of Piper betle which is one of the family Piperaceae.