Danum Valley is found at about 100 m a. s. l. in Sabah and represents on the list of significant remaining patches of Sabahs key lowland rainforest. It has a standard equatorial rainforest climate with a mean an nual temperature of 27 C and a yearly rainfall of 2700 mm. We studied parabioses of Crematogaster modiglianii and Camponotus rufifemur, which nest in hollow tree trunks, Camponotus rufifemur happens in two chemically and genetically distinct varieties, which likely represent unique species, In con trast, the profiles on the connected Cr. modiglianii had al most no compounds in prevalent with Ca. rufifemur and did not display differentiation into chemical types, with two exceptions. 27 MeC39 14 ene and 27 MeC39 sixteen ene, the key surface components of the red Ca. rufifemur, occurred in individuals Cr. modiglianii colonies that lived in parabiosis with all the red Ca.
rufifemur but was absent from other people, Behavioural experiments have been carried out selleck chemical with two nests and one particular employee colony that had been brought for the laboratory at Danum Valley, in which each species had been kept together inside a part of their ori ginal nest trunk in a Fluon covered plastic box for ca. two months or few days, respectively. For your experiments, more ants had been collected from four supplemental nests, Here, we use the phrase nest for entire nests inside a liv ing tree trunk, when worker colony refers to groups of employees that have been caught at a parabiotic nest. In accordance towards the Ca. rufifemur range, the parabiotic colonies will be labelled B or R plus a digit in the following. Chemical analyses We analysed the novel compounds from Cr.
modiglianii cuticular extracts employing electron ionization mass spectrom etry, chemical ionization mass spectrometry, substantial resolution mass spectrometry, and nuclear magnetic resonance, In addition, many de rivatizations have been carried out and subsequently analysed with GC MS, First of all, we characterized various substances and their relative quantities inhibitor Cyclopamine from 7 Cr. modiglianii colonies by their electron ionization mass spec tra. Extracts were obtained from twenty 90 folks per ex tract, and we analysed one 8 extracts per colony. We carried out capillary gasoline chromatography mass spectrom etry that has a Hewlett Packard 6890 series fuel chro matograph coupled to a HP 5973 Mass Selective Detector. The GC was outfitted with a J W Scientific DB 5 fused silica capillary column, The temperature on the GC was stored at 60 C for two min, then increased by 60 C min up to 200 C and subsequently by 4 C min to 320 C, wherever it remained frequent for ten min. The transfer line had a temperature of 325 C. He lium was applied as carrier gas having a constant movement of one. 0 ml min. A split splitless injector was set up at 250 C while in the splitless mode for thirty s.