Several strong bands were detected by our Western blot analysis of the rat brain nuclear protein at around 26 kDa and 31 kDa as well as some high molecular weight bands. which while they occur in the blot in which Bax antisera were neglected, appear to be due to low specific binding of the secondary antiserum Fig. 5.. Specific groups were found at around 42 kDa Deborah 20 Bax. and 45 kDa PC66 Bax. and P 19. In the control individual brains we found moderate staining of N 20 Bax in the granule cell layer of the hippocampus in 7r8 cases. The staining seemed to be nuclear, and was entirely abolished by pre absorption with the N 20 Bax peptide Fig. 6.. We found a substantial p 0. 05. decrease in N 20 and P 19 Bax discoloration in-the dentate granule cells of AD pieces weighed against the settings Figs. Staining in the HD granule cells was paid down, however not significantly different from the controls Fig. 7.. Neurofibrillary tangles were clearly Deborah 20 Bax. or weakly P PC66 Bax and 19. 6., and D 20 Bax staining was abolished by pre consumption with the N 20 Bax peptide. Additionally, the N 20 and PC66 antisera Ribonucleic acid (RNA) noticed Bax in t amyloid positive senile plaques in AD brains in the same structure to the staining observed using antisera to t protein Fig. 6., again the N 20 Bax staining was eliminated by pre consumption using the N 20 Bax peptide. Using G 19 Bax we observed intense staining in senile plaques in most AD cases except AZ15 which doesn’t exhibit any bamyloid staining in-the hippocampus. in a way similar to t amyloid staining in these heads Fig. 6.. That antisera also detected staining in astrocyte like cells, which Letrozole CGS 20267 in one single case AZ22. were associated with plaque like structures, in AD brains Fig. 6.. All three Bax antisera stained pyramidal cells, with the staining mainly perinuclear including dendritic processes, and several cases displaying nuclear staining as well as this Fig. 8.. Pyramidal cell staining was usually limited to small specific parts of the pyramidal cell layer, with no clear pattern and no clear difference in staining in the CA1 region a significant area of cell loss in the hippocampi of AD brains w83x. between AD brains and controls. Macrophages connected with the granule cell layer and microcapillaries were stained by the N 20 antiserum in certain control, HD and AD cases, but this was not abolished by pre consumption with the N 20 Bax peptide.