It is a 75–80-kDa disulfide-linked heterodimeric protein

with about 30% of the mass of the molecule comprised of N-linked carbohydrate which is branched, complex, and rich in sialic acid [10]. Clusterin is an enigmatic molecule, implicated in diverse biological processes, and has additionally been associated with opposing functions in regard to apoptosis [11]. Possible protective mechanisms are considered by blockage of the terminal complement cascade (C5b-9) or by protecting against oxidative stress [12] and [13]. CH5424802 chemical structure More recent studies show that clusterin may be a secreted chaperone molecule, inhibiting stress-induced precipitation of a very broad range of structurally divergent protein substrates and binding irreversibly via an ATP-independent mechanism

to stressed proteins to form solubilized high molecular weight complexes GW-572016 solubility dmso [14] and [15]. The first aim of this study was to determine levels of clusterin in pediatric patients with systemic inflammatory response syndrome (SIRS) or septic state, comparing these levels with a healthy population. The second objective was to compare levels of clusterin within individual septic conditions, and influence of levels of this protein on mortality. Prospective observational study occurred during the period from June 2009 to March 2011. The study protocol and informed consent approach were approved by the Ethics committee of the University Hospital, Brno. Parents provided informed written consent for their children to participate in this trial. Data were collected and analyzed from fifty-seven consecutive patients with SIRS or septic state who were admitted to the Department of Anesthesia and Intensive Care of the University Children’s Hospital Brno, Czech Republic. The most common sources of infection that led to sepsis were the lungs – bacterial and viral infections, and central nervous system – bacterial infections of the brain. Infections, sepsis, severe sepsis, septic shock and multiple

organ dysfunction syndrome (MODS) were defined according PLEK2 to commonly used criteria – by International pediatric sepsis consensus conference. The criteria for adult SIRS were modified for pediatric use. Age-specific norms of vital signs and laboratory data were incorporated into the definitions of SIRS. Sepsis was defined as SIRS associated with suspected or proven infection [16]. Patients were categorized into five groups according to their clinical data and to the described definitions: (a) SIRS, (b) sepsis, (c) severe sepsis, (d) septic shock, (e) MODS. In these groups, we compared the difference in the levels of clusterin. The samples from 70 children undergoing elective surgery were used as controls (strabismus surgery, umbilical and inguinal hernia repair), i.e. samples from patients without signs of infection. Blood samples were collected before surgery.