Methods: Twenty male SD rats were randomly divided into 2 Cell Cycle inhibitor groups:
the model group (n = 10) and the control group (n = 10). Rats in the model group were treated with chronic and comprehensive stress. Open-field test was used to confirm the accomplishment of modeling. The serum concentration of IL-4 and IL-13 were determined by ELISA and the expression of TMEM16A in the myenteric plexus was detected by immunofluorescence. Results: The mean serum concentration of IL-4 in the model group (8.09 ± 0.92 pg/ml) was significantly higher than that in the control group (6.98 ± 0.69 pg/ml) (t = 3.363, P < 0.01), while the mean serum
concentration Akt inhibitor of IL-13 in the model group (5.96 ± 0.67 pg/ml) was also significantly higher than that in the control group (5.26 ± 0.73 pg/ml) (t = 2.322, P < 0.05). Positive expression of TMEM16A in the myenteric plexus was observed under the fluorescence microscope. Compared with the control group, the expression of TMEM16A in the model group were decreased in all sections of the intestine. Conclusion: In model rats treated with chronic and comprehensive stress, the expression of Th2-related cytokines (IL-4 and IL-13) were increased, and it might result in the damage of interstitial cells of Cajal by affecting the expression of TMEM16A through the JAK/STAT pathway. Key Word(s): 1. IL-4;
2. IL-13; 3. TMEM16A; 4. ICC; Presenting Author: FENGPING ZHENG Additional Authors: SHENGLIN WEN, LI TAO Corresponding Author: FENGPING ZHENG Affiliations: The third Affiliated Hospital of Sun Yat-Sen University Objective: To evaluate the relationship of the irritable bowel syndrome (IBS) with life events and social support. Methods: The life event scale (LES) and social support rating scale (SSRS) were applied for investigating eighty-three patients with IBS and seventy-six MCE healthy control respondents. Results: The score of negative life events was higher in the IBS group than the control group (21.2 ± 17.4 vs. 9.5 ± 11.0, P < 0.05). The score of positive life events and total stress in the IBS group was not significantly different from the control group (P > 0.05). Compared with controls, the social support offered to IBS patients was lower (37.6 ± 7.2 vs. 43.9 ± 4.8, P < 0.05) and IBS patients utilization of social support was also lower (5.6 ± 3.2 vs. 8.2 ± 2.7, P < 0.05). Conclusion: IBS patients experienced a higher level of negative life events and acquired a lower level of social support compared with healthy control respondents. Key Word(s): 1. IBS; 2. life event; 3.