On the other hand, galE (KP02995) was identified outside the cps region, and it learn more encodes a UDP-glucose 4-epimerase with roles in the amino sugar and nucleotide sugar pathways producing UDP-D-galactose from UDP-D-glucose (Figure 3). The presence of this gene suggests that the capsule composition of Kp13 could also include UDP-D-galactose derivatives. Neither the manA, manB and manC genes of the cps cluster nor other genes of the mannose and fucose biosynthesis pathways were identified in the Kp13 genome. This suggests that the CPS of Kp13 does not contain GDP-D-mannose or GDP-L-fucose derivatives. Proteins involved in translocation, surface assembly

and polymerization: Wzi, Wza, Wzb, Wzc, Wzx and Wzy The deduced amino acid sequences of the wzi and wza genes found in cps Kp13 show 98% and 97% identity, respectively, with homologs from K. pneumoniae VGH484 Savolitinib clinical trial (Table 1), and both proteins were predicted to localize in the outer membrane (PSORTb scores: Wzi, 9.52; Wza, 9.92). Moreover,

a signal Cediranib peptide was predicted for the wzi gene product. Analysis of the secondary structure of the Kp13 Wzi protein using PSIPRED showed that it is rich in β-sheet regions (data not shown), an observation that has been experimentally confirmed for a Wzi homolog in E. coli [GenBank:AAD21561.1] [20] which shares 98% identity with that of Kp13. Also, Rahn et al. [20] established the importance of the Wzi outer membrane protein for capsule synthesis by showing that wzi mutants have lower amounts of cell-associated capsular polysaccharide. Isotretinoin The wza product of Kp13 has 92% identity with Wza from E. coli [GenBank:AAD21562.1], which has been shown to be an integral lipoprotein with exposed regions on the cell surface. The E. coli protein forms a ring-like structure responsible for polymer translocation through the outer

membrane [12]. Wzc and Wzb are a tyrosine autokinase and its cognate acid phosphatase, respectively, and they are ubiquitously found in group 1 capsule clusters [12, 21]. The Kp13 Wzc protein was predicted to have two transmembrane regions, like its counterpart in the K. pneumoniae strain Chedid, with which it shares 72% amino acid identity [Swiss-Prot:Q48452]. The inner membrane is the probable location of Kp13’ Wzc (PSORTb score 9.99), in agreement with its role in capsule synthesis. Wzc is involved in the translocation of capsular polysaccharide from the periplasm to the cellular surface through formation of a complex with Wza [22]. Wzc undergoes autophosphorylation of its tyrosine-rich C-terminal residues (of the last 17 residues in Kp13 Wzc, eight are Tyr) potentially modulating the opening and closing of the translocation channel [12]. The Wzb protein (EC 3.1.3.48) of Kp13 is probably located in the cytoplasm (PSORTb score: 9.26). Wzb catalyzes the removal of a phosphate group from phosphorylated Wzc and is necessary for continued polymerization of the repeat units [12].