ostreatus ( Nunes et al., 2012). Thus, the contents of phorbol ester and antinutritional factors found in jatropha seed cake do not inhibit the fungal growth and mushrooms production. Besides, selleck we did not observe any morphological changes in the mushrooms. The nutritional composition of the mushrooms produced in J. curcas seed cake showed that this food is a source of protein, carbohydrates, phosphorus and ergosterol ( Table 1). The contents of these nutrients were similar to those found in P. ostreatus mushroom grown in different agroindustrial residues ( Dundar et al., 2009; Nunes

et al., 2012; Tewari, 1986; Wang et al., 2001). According to Tewari (1986), the mushrooms contain 85 (g/100 g) to 95 (g/100 g) water, 3 (g/100 g) protein, 4 (g/100 g) carbohydrates, and 1 (g/100 g) minerals and vitamins. However, these nutrient contents, mainly the proteins, depend on the substrate composition ( Dundar et al., 2009).

Potassium, phosphorus, ZD6474 clinical trial copper, iron and calcium are the main minerals found in mushrooms ( Wang et al., 2001). P. ostreatus mushrooms are also rich in amino acids, fibers and vitamins, including thiamine, riboflavin, pyridoxine and niacin ( Dundar et al., 2009; Wang et al., 2001). The ergosterol content found in the P. ostreatus mushroom ( Table 1) was greater than the content of this compound observed in commercial mushrooms by Jasinghe and Perera (2005). In the P. ostreatus mushrooms of this study, neither tannins nor phytic acid were detected ( Table 1), but low levels of phorbol ester were found. The concentration of this compound decreased in function of incubation time ( Table 2). Furthermore, phorbol ester concentration of the mushrooms ( Table 2) was around 1000-fold lower than the concentration of this compound found in the non-toxic variety of J. curcas ( Makkar et al., 1998). This Mexican

variety has 0.11 mg/g of phorbol ester and was not toxic to fish, chickens or rats ( Makkar et al., 1997). According to these authors, the seeds of this non-toxic variety were typically consumed by humans and chickens. Phorbol ester concentrations of 0.8 mg/g or higher caused 3-mercaptopyruvate sulfurtransferase appetite loss, diarrhea and reduced motor activity in rats fed with the seed meal Jatropha ( Rakshit, Darukeshwara, Raj, Narasimhamurthy, Saibaba, & Bhagya, 2008). As phorbol ester content in the mushrooms was from 0.009 to 0.081 μg g−1 ( Table 2), it could be used as food, even so, we suggest to test in animals to guarantee the food security. In this study we show the potential to use the residue of biodiesel to produce mushroom, a food with high nutritional value. Also, the antinutritional factors degradation allows using this residue as animal feed, adding economic value and avoiding inadequate disposal in the environment.