rufifemur colony and ex tracts of Crematogaster difformis and Crematogaster coriaria. Surface extracts for your behavioural assays had been obtained by immersing 50 freeze killed ants in hexane for ten minutes. Non polar and polar fractions of these ex tracts were eluted with distilled hexane, followed by chloro kind, utilizing conditioned SiOH columns, GC MS ana lyses confirmed the hexane fractions contained only hydrocarbons when the chloroform fractions contained solely the novel compounds. The chloroform from the polar fraction was evaporated, as well as fractions were reconstituted in hexane. As dummies we made use of intracolonial bodies of Cr. modiglianii or Ca. rufifemur that had been extracted with 4 ml hexane, four ml chloroform, 4 ml chloro form, and 4 ml hexane for ten min each and every.
Just about every dummy was treated with an extract amount equivalent to 5 individ uals. This going here amount was picked to account for potential substance losses in the course of extraction and fractionation. the volume had effectively elicited differential behavioural re actions in earlier experiments, Note that, from the crematoenone addition experiments, the same absolute quantities of hydrocarbons were transferred onto a dummy after with and after with out crematoenones. To the Ca. rufifemur assays, a black Ca. rufifemur colony was confronted with therapies from intracolonial Cr. modiglianii and an allocolonial Cr. modiglianii, Secondly, a black Ca. rufifemur employee colony was confronted with intracolonial Cr. modiglianii and allocolonial Cr. modiglianii remedy series. Lastly, a red Ca. rufifemur colony was confronted with Cr.
modiglianii deal with ments from your identical and 3 other colonies, For the Cr. modiglianii assays, we confronted a Cr. modiglianii colony with intracolonial and two allocolonial treatments, Within the Ca. rufifemur assays, the Cr. modiglianii dum mies have been small compared to Ca. rufifemur employees. For that reason, we successively held them in front of 10 single employees that Dovitinib have been walking around from the nest, and categorized every person reactions as peaceful, weakly or strongly aggres sive, From the Cr. modiglianii assays, the Ca. rufifemur dummy was held with forceps onto the nest trunk inside the plastic box to ensure that various Cr. modiglianii ants could interact with it concurrently. In the course of 3 minutes, each observed interaction was then recorded and classified as above. The observer was not blind on the respective therapies, but was unaware of the hypotheses currently being examined. Inside these 3 minutes, continued interactions had been recorded yet again each ten sec to provide additional excess weight to prolonged lasting interactions. This strategy is steady with an earlier review, All vary ent treatments had been examined in haphazard buy and on distinctive places in the nest trunk.