Studies examining the effects of C646 calcium intake and level of physical activity in free living conditions on bone mineralization are also limited, particularly in
young men. In addition, intake of dairy products, which are the main source of calcium , may be associated with a dietary fat intake  and adversely affect blood lipids  or blood pressure. Only one study with girls examining effect of calcium and bone mineralization has investigated the effects of calcium intake on blood lipids. This study aimed to examine the relationship between dietary factors, physical activity and bone mineralization in young men. Blood lipids were also assessed in the P505-15 current study. Methods Thirty-five healthy men aged 18–25 y, recruited from the local community in the city of Brisbane, Australia volunteered for the study. Participants were recruited by flyers posted in shopping centers and education centers as NVP-BSK805 research buy well advertisement in local newspapers. Inclusion criteria to participate in
the study were age between 18 and 25 years and absence of any chronic disease. Queensland University of Technology Human Research Ethics Committee approved the participant recruitment and data collection procedures. The methods of this cross-sectional study have been previously described in detail  and are here described in brief. Anthropometric measures including body weight and height, body composition, and waist and hip circumferences were undertaken. Body mass index (BMI) was calculated as weight (kg) divided by height (m2). Body composition, including BMC, BMD and lean body mass, was measured by dual-energy X-ray absorptiometry (DXA) (DPX-Plus; Lunar Corp, Madison, WI). Resting metabolic MYO10 rate (RMR) was assessed by continuous open-circuit indirect calorimetry using a Deltatrac II metabolic cart (Datex-Ohmeda Corp., Helsinki, Finland http://www.hospitalnetwork.com/doc/Deltatrac-II-Metabolic-Monitor-0001)
in half of the participants. Due to technical problems, the MOXUS O2 system (AEI Technologies, Pennsylvania, USA) was used to assess RMR of the remaining participants. In our laboratories we have consistently found measured RMR values are less than 100 kcal lower using the Deltatrac compared to MOXUS system. A similar proportion of lean and overweight participants were assessed using each of the methods and therefore likelihood of measurement bias was reduced. Sitting blood pressure (BP) was assessed after a 10-min rest using a standard sphygmomanometer. Following an overnight fast of at least 8 h, a blood sample was collected for later total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and triglycerides (TG) determination using reagents from Roche Diagnostics (Indianapolis, IN).