The diamond film thickness is 300�C400 nm. Average crystal size is 50 nm, RMS roughness at 1��1 ��m2 area is 15�C20 nm as measured by AFM using standard silicon tips of nominal radius < 10 nm. The silicon substrates are coated with NCD film on both sides, silicon is thus hermetically encapsulated in the diamond.The diamond films were further chemically cleaned EtOH in acids (97.5% H2SO4 + 99% powder KNO3) at 200��C for 30 minutes. The surface was then hydrogenated at 800��C for 10 min. Water wetting angle on H-terminated diamond was 80��. NCD films were lithographically processed to generate alternating H- and O-terminated patterns of 30 to 200 ��m widths. A positive photoresist ma-P 1215 (micro resist technology GmbH, Germany) was applied.

NCD films with the lithographic masks were treated in oxygen radio-frequency plasma (300 W power, 3 min process time) to oxidize the surface and hence to generate the hydrophilic patterns. Then the sample was rinsed in a stripper, de-ionized water and dried. This process removed possible surface contamination [26]. The H/O-termination quality was proved by a scanning electron microscope Inhibitors,Modulators,Libraries (SEM; JEOL Superprobe 733). Electronic measurements detected a surface conductivity of 10?5 S/sq on the H-terminated surfaces [27]. Surfaces with O-termination were highly resistive. Water wetting angle on O-terminated diamond was 20��. The NCD samples were sterilized in 70% ethanol for 10 minutes prior to the cell plating. The device concept is schematically shown in Figure 1.Figure 1.

Schematic picture of silicon substrate hermetically coated with diamond layer with stripe-like patterns having hydrogen or oxygen surface termination. Cell adhesion on the O-terminated region is also schematically indicated.SAOS-2 cells (human osteoblast-like cell line) (DSMZ GmbH), were grown in McCoy’s 5A medium (BioConcept) supplemented with heat inactivated fetal Inhibitors,Modulators,Libraries bovine serum (FBS; Biowest) of various concentrations (0-15%), penicillin (20 U/mL) and streptomycin (20 ��g/mL). We have used osteoblasts because SAOS-2 Inhibitors,Modulators,Libraries is a standard immortalized cell line which keeps constant Inhibitors,Modulators,Libraries properties during a long period of time. Thus the results can be compared between various series of experiments as well as with reports in the literature. Osteoblasts’ response to Entinostat different materials and in different time periods is well studied, thus we can draw conclusion also from experiments using new materials (diamond) and experimental setups.

Osteoblasts are cells generating bone so they are also relevant for osseointegration applications. Cells were plated in the densities of 2,500 and 10,000 Volasertib chemical structure cells/cm2 using a droplet technique: substrate surface was covered by 100 ��L droplet of cell suspension in the appropriately supplemented medium, let to incubate for 2 h (adhesion time), and then 1.4 mL of the medium was added. In the case of 0% FBS, the cells were plated and incubated for 2 h in the medium without the serum.