Figure 11 Camera lucida drawings of two pyriform (A1) and (A2) and two pyramidal (B1) and (B2) neurons from different callosal regions. Dendrites from neurons in A2 and B1 reach the overlying white matter (wm). Calibration bar: 50 μm. Triangular neurons were about 10.70% (see Table ​Table3)3) and had a thick and wavy apical dendrite from which originated thin secondary spiny Bosutinib mouse dendritic branches (Fig. ​(Fig.11B-1).11B-1). Apical dendrites crossed the cc in the direction of the white matter, sometimes reaching it. The dendrites of some triangular Inhibitors,research,lifescience,medical neurons were completely devoid of spines. The morphology of the intracallosal neurons whose dendrites reached the cerebral cortex could be studied

in very few cases, as the extreme thinness of the distal dendrites and poor labeling made it difficult to follow their course. Overall 76 neurons were studied in cases CC-NADPH-5, -7, -9; of these

33 (43.42%) were polygonal, 17 (22.36%) were pyramidal, 15 were round (19.73%), and 11 were fusiform (14.47%). Inhibitors,research,lifescience,medical NO-producing neurons and blood vessels NADPH-d+/NOSIP elements were closely associated with cc blood vessels (Fig. ​(Fig.6B,6B, C, E, and F, Fig. ​Fig.12).12). Counts performed in three NADPH-d experiments (NADPH-d-5; -7; -9; see Table ​Table4)4) suggest that this population accounts Inhibitors,research,lifescience,medical for about 38% of all intracallosal NO-producing neurons. The most common morphological types close to blood vessels were polygonal (29.42%; see Table ​Table4)4) and bipolar neurons (28.15%; see Table ​Table4).4). Inhibitors,research,lifescience,medical Nerve fibers presenting varicosities along their course were detected in the adventitial layer of blood vessels, where they sometimes formed a tangled network (Fig. ​(Fig.12).12). Intensely labeled neurons were observed over or very near the vascular profiles, their cytoplasmic profiles wrapped Inhibitors,research,lifescience,medical around the walls of cc vessels (Fig. ​(Fig.12A,12A, B, D, and F; for NOSIP neurons see Fig. ​Fig.5D).5D). In other cases, intensely labeled cc neurons, at a distance from cc

vessels, emitted long cytoplasmic fibers that could be followed as far as the neighboring blood vessel, where they formed a dense network (Fig. ​(Fig.6B;6B; Fig. ​Fig.12C12C and F). Often positive cytoplasmic processes found along intracallosal blood vessels were not attributable to any of the surrounding neurons. Figure 12 Photomicrographs showing NADPH-h+ neurons lying close to blood vessels. (A), (D) NADPH-d+ Suplatast tosilate neurons in the splenium of the corpus callosum. Cell bodies and their processes are closely apposed to the wall of a longitudinal blood vessel. Photomicrographs … Double-labeling experiments (CC-Fl-1 and -2) Colocalization of nNOS and GFAP was studied in two rats using confocal microscopy. Green fluorescent nNOSIP neurons (Fig. ​(Fig.13B)13B) showed morphological and distribution features consistent with those described in the preceding paragraphs.