Granular bodies of approximately 35 nm in diameter were observed in the spaces between the parallel lamellae of the main rod (Figure 5B). The ventral side

of the main rod was embedded in an amorphous matrix that became thinner toward the posterior end of the cell, until it disappeared altogether (Figure 6A-D). A single row of longitudinal microtubules lined the external side of the main rod, which delimited the boundary between the main rod and the accessory rod for most of their length (Figure 5A-B). Figure 5 Transmission electron micrographs (TEM) of non-consecutive serial sections of Bihospites bacati n. gen. et sp. through the vestibular selleckchem region of the cell. A. TEM showing the nucleus (N) with

condensed chromatin, the dorsal side of the C-shaped rod apparatus consisting of the main rod (r) and the accessory rod (ar), and the vestibulum (vt). Several rod-shaped bacteria (black arrows) and spherical-shaped bacteria line inner surface of the vestibulum (vt) (bar = 10 μm). B. High magnification view of the C-shaped rod apparatus in Figure A showing the single row of microtubules (arrowheads) positioned at the junction between the tightly connected rod and accessory rod. Granular bodies (arrows) are present between the parallel lamellae Z-VAD-FMK order that form the main rod (bar = 500 nm). C, D. Transverse TEMs showing the cytostomal funnel (cyt) and two separate lobes of the feeding pocket (arrowheads). Bacterial profiles can be seen inside the feeding pocket (arrows). Figure D uses color to distinguish between the feeding pocket (red), the vestibulum (blue), and the two branches of the flagellar pocket (green). E, F. Transverse TEMs at a more posterior level than in Figure C-D showing the posterior end of the main C-shaped rod (arrow) emerging within the posterior end of the feeding Nintedanib (BIBF 1120) pocket. The cytostomal funnel (arrowheads) opens and fuses with the feeding

pocket. Figure F uses color to distinguish between the feeding pocket (red), the vestibulum (blue), and the two branches of the flagellar pocket (green). (C-F bar = 2 μm). Figure 6 Transmission electron micrographs (TEM) of non-consecutive serial sections through the flagellar apparatus and feeding pockets of Bihospites bacati n. gen. et sp. TEMs taken at levels posterior to those shown in Figure 5 and presented from anterior (A) to posterior (D). A. TEM showing the posterior end of the main C-shaped rod (r) embedded in an amorphous matrix (double arrowhead) and surrounded by a thick membrane with fuzzy material (arrowhead). At this level, the rod is associated with ‘congregated globular structure’ (CGS), and the striated fibres that form the accessory rod (ar) appear near the cytostomal funnel (cyt) at the junction between the feeding pocket and the flagellar pocket.