In the present study, owing to the metabolism of the trace elements is altered in either infection or inflammation, we explored the treatment of H. pylori infection and its association with the changes of some serum essential trace elements for the first time. Methods: Subjects

were collected according to the treatment and non-treatment for H.pylori. Patients Saracatinib treated with amoxicillin and clarithromycin were classified as first line treatment, while the patients treated with bismuth, tetracycline and metronidazole were classified as second line treatment. The final treatment used bismuth, amoxicillin and levofloxacin were classified as third line treatment. Every group consisted of twenty subjects. Essential trace elements including iron, copper, zinc, and selenium were analyzed using high sensitive Inductively coupled plasma mass spectrometry. Results: Our major findings indicated that a significant difference (p < 0.05) in serum iron concentration was observed in second line treatment when compared with those of first line treatment. Also, our results showed that the third line treatment would have higher serum iron concentration than those of the control group with significant difference (p < 0.05). In addition, a significant difference (p < 0.05) in serum LBH589 zinc concentration was observed in second line treatment when compared with those of first line treatment. Conclusion: Some

serum essential trace elements such as iron and zinc might play certain

roles in the evaluation of the treatment efficiency of H.pylori. Further study could devote to explore the existence of H.pylori and its relationship with iron and /or zinc. Key Word(s): 1. Helicobacter pylori; 2. Iron; 3. Zinc; Presenting Author: YANYAN SHI Additional Authors: LINNA LIU, YUEXIA ZHANG, selleck products XIANGMEI CHEN, TING ZHANG, JING ZHANG, YE WANG, SHIGANG DING Corresponding Author: SHIGANG DING Affiliations: Peking University Third Hospital; Peking University Health Science Center Objective: Helicobacter pylori (H. pylori) infection has been proved to be related to the development of gastric diseases, but the pathogenic mechanism has not been clear. We want to identify the pathogenic properties of H. pylori, and to investigate the identified protein. Methods: H. pylori strains were isolated from endoscopic biopsy specimens of gastric mucosa from patients with gastric cancer, peptic ulcer, and gastritis. The proteins were analyzed by two-dimensional gel electrophoresis and mass spectrometry. Then the expressions of Trx1 were analyzed by real-time PCR. H. pylori expressing high or low Trx1 levels was co-cultured with gastric cancer cell line BGC-823 and gastric epithelial cell line GES-1 respectively. MTT, cell cycle and cell apoptosis were used to estimate cell growth. Western blot was used to measure the related proteins. The two strains were used to make chronic animal models by infecting Mongolian gerbils for 91 weeks. Results: Trx1 expression of H.